Journal: Brain : a journal of neurology

Article Title: Connexin43 mimetic peptide reduces vascular leak and retinal ganglion cell death following retinal ischaemia.

doi: 10.1093/brain/awr338

Figure Lengend Snippet: Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant endothelial cell death in vitro in rat brain microvascular endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.

Article Snippet: Rat brain microvascular endothelial cells (R840K-05a, Cell Applications) were plated into 24-well plates (1 105 cells/well) in rat brain microvascular endothelial cell growth media (R819K-500, Cell Applications) and allowed to settle for 16 h. Medium was then removed and replaced with Dulbecco’s Modified Eagle’s Medium/F12 containing 0.5% foetal bovine serum and 1% glutamine.

Techniques: In Vitro, Control

Journal: Drug Metabolism and Disposition

Article Title: Activity, Inhibition, and Induction of Cytochrome P450 2J2 in Adult Human Primary Cardiomyocytes

doi: 10.1124/dmd.113.053389

Figure Lengend Snippet: Kinetic parameters of terfenadine and astemizole metabolism using recombinant enzyme or cardiomyocytes

Article Snippet: Adult-derived primary human cardiomyocytes, cell culture media (complete growth media and serum-free media), solutions, and cell culture materials (culture flasks and plates, precoated with proprietary matrix for cell adherence) were purchased from Celprogen Inc. (San Pedro, CA).

Techniques: Recombinant

Journal: Drug Metabolism and Disposition

Article Title: Activity, Inhibition, and Induction of Cytochrome P450 2J2 in Adult Human Primary Cardiomyocytes

doi: 10.1124/dmd.113.053389

Figure Lengend Snippet: Relative levels of mRNA expression in human cardiomyocytes and human ventricular heart tissue.

Article Snippet: Adult-derived primary human cardiomyocytes, cell culture media (complete growth media and serum-free media), solutions, and cell culture materials (culture flasks and plates, precoated with proprietary matrix for cell adherence) were purchased from Celprogen Inc. (San Pedro, CA).

Techniques: Expressing

Journal: Drug Metabolism and Disposition

Article Title: Activity, Inhibition, and Induction of Cytochrome P450 2J2 in Adult Human Primary Cardiomyocytes

doi: 10.1124/dmd.113.053389

Figure Lengend Snippet: Kinetic parameters of terfenadine hydroxylation (A) and astemizole demethylation (B) in human cardiomyocytes.

Article Snippet: Adult-derived primary human cardiomyocytes, cell culture media (complete growth media and serum-free media), solutions, and cell culture materials (culture flasks and plates, precoated with proprietary matrix for cell adherence) were purchased from Celprogen Inc. (San Pedro, CA).

Techniques:

Journal: Drug Metabolism and Disposition

Article Title: Activity, Inhibition, and Induction of Cytochrome P450 2J2 in Adult Human Primary Cardiomyocytes

doi: 10.1124/dmd.113.053389

Figure Lengend Snippet: Inhibition of terfenadine hydroxylation at 0.2 μM (A) and 1.5 μM (B) at 1-μM and 10-μM inhibitor concentrations after 2 hours of incubation in human cardiomyocytes.

Article Snippet: Adult-derived primary human cardiomyocytes, cell culture media (complete growth media and serum-free media), solutions, and cell culture materials (culture flasks and plates, precoated with proprietary matrix for cell adherence) were purchased from Celprogen Inc. (San Pedro, CA).

Techniques: Inhibition, Incubation